Premium
Epithelioid osteosarcoma of bone immunocytochemical evidence suggesting divergent epithelial and mesenchymal differentiation in a primary osseous neoplasm
Author(s) -
Kramer Kim,
Hicks David G.,
Oppenheimer Jonathan,
Palis James,
Rosier Randy N.,
Fallon Michael D.,
Cohen Harvey J.
Publication year - 1993
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19930515)71:10<2977::aid-cncr2820711015>3.0.co;2-u
Subject(s) - vimentin , histogenesis , cytokeratin , osteosarcoma , pathology , keratin , mesenchymal stem cell , immunohistochemistry , alkaline phosphatase , biology , medicine , biochemistry , enzyme
Background . The combination of a primary osteosarcoma of bone with a second carcinomatous cell type has been recognized, although immunohistochemical studies currently have not been performed in an attempt to understand the histogenesis of such a tumor. Methods . In this report, the authors performed immunohistochemical studies on a primary osseous carcinosarcoma. Using a biotin‐streptavidin peroxidase conjugate technique, the expression of keratin, epithelial membrane antigen, and vimentin was analyzed. Results . The epithelial cells expressed cytokeratin and epithelial membrane antigen but did not express vimentin. The mesenchymal cells strongly expressed vimentin, and only rare cells expressed cytokeratin. Conclusions . The clinical, morphologic, and immunophenotypic data in this instance strongly suggest divergent differentiation of a primitive multipotential uncommitted stem cell in a primary osseous tumor.