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Isolation of Inoue‐Melnick virus from colorectal carcinoma tissues
Author(s) -
Ito Michio,
RodriguezBigas Miguel A.,
Evans Mary Jo,
Petrelli Nicholas J.
Publication year - 1993
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19930315)71:6<1960::aid-cncr2820710606>3.0.co;2-7
Subject(s) - colorectal cancer , medicine , carcinoma , pathology , cancer
Background . Isolation of Inoue‐Melnick virus (IMV) Type 1 from tumor tissues of patients with colorectal carcinoma in Japan was reported. A high prevalence of IMV antibodies has been found among patients with colorectal carcinoma in Buffalo, New York. Methods . IMV was isolated from homogenates of colorectal carcinoma and other tissues, inoculated into MRC‐5 human fibroblast cultures. IMV‐specific cytopathic effects were detected and titrated following 7 days of incubation in a roller‐tube apparatus. Results . IMV was isolated from all nine (100%) colorectal carcinoma tissues tested and all four (100%) normal parts of colon mucosa tissues obtained from patients with cancer. Biopsy specimens of normal colon mucosa from 10 patients without colon carcinoma were negative for IMV (0%). Average infectious titers of IMV in these tissue homogenates were 4.41 for tumor tissue and 2.55 log 10 tissue culture infectious dose by 50% (TCID 50 )/0.1 ml for colon mucosa, respectively. Most of the isolates were the IMV intermediate type. Conclusions . Our results confirmed the production of infectious IMV in colorectal carcinoma tissues, as reported recently in Japan. The role of IMV in colorectal carcinogenesis is currently unknown.