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Synergistic antiproliferative activity of human fibroblast interferon in combination with alpha‐difluoromethylornithine against human gastric cancer cells In vitro
Author(s) -
Kubota Shunichiro
Publication year - 1992
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19920515)69:10<2395::aid-cncr2820691002>3.0.co;2-h
Subject(s) - ornithine decarboxylase , in vitro , polyamine , cell growth , medicine , growth inhibition , alpha interferon , fibroblast , cancer cell , interferon alfa , cancer , pharmacology , cancer research , interferon , microbiology and biotechnology , enzyme , biology , immunology , biochemistry
The author has studied the effects of alpha‐difluoro‐methylornithine (αDFMO), an enzyme‐activated irreversible inhibitor of ornithine decarboxylase; human fibroblast interferon (IFNβ); and their combination on human gastric cancer cell growth in vitro. αDFMO (from 0.1 to 4 mmol/l) inhibited cell growth in a dose‐dependent manner. Both αDFMO (0.1 mmol/l) and higher doses of IFNβ (100 and 1000 IU/ml) caused only limited inhibition of cell growth. When αDFMO (0.1 mmol/l) was administered in combination with IFNβ (100 and 1000 IU/ml), synergistic antiproliferative activity was observed 7 days after continuous exposure. Although the mechanisms by which this effect occurs are unclear, it appears to be associated with direct inhibition of tumor cell proliferation, possibly by modulation of polyamine metabolism. Cancer 1992; 69:2395‐2399.