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Establishment and characterization of a new human extrahepatic bile duct carcinoma cell line (KMBC)
Author(s) -
Yano Hirohisa,
Maruiwa Masafumi,
Lemura Akihiro,
Mizoguchi Atsushi,
Kojiro Masamichi
Publication year - 1992
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19920401)69:7<1664::aid-cncr2820690705>3.0.co;2-p
Subject(s) - carcinoembryonic antigen , alkaline phosphatase , bile duct , carcinoma , antigen , adenocarcinoma , pathology , cell culture , doubling time , immunoperoxidase , bile duct carcinoma , medicine , biology , immunology , antibody , cancer , enzyme , biochemistry , genetics , monoclonal antibody
A new human extrahepatic bile duct carcinoma cell line (KMBC) was established from a serially transplanted tumor in nude mice that originated from a surgically resected tumor from a 73‐year‐old Japanese man; the cell line has been maintained for 5 five years. KMBC cells proliferate in a monolayered sheet with a population doubling time of 30 hours. Chromosome number was distributed in a range from 37 to 44, with modal numbers of 40 and 41. KMBC cells and the reconstituted tumor in a nude mouse showed moderately to poorly differentiated adenocarcinoma and possessed various functional characteristics of extrahepatic bile duct carcinoma. KMBC cells secreted carbohydrate antigen 19‐9, tissue polypeptide antigen, carcinoembryonic antigen, ferritin, β 2 ‐microglobulin, fibronectin, and α 2 ‐macroglobulin and produced glutamic oxaloacetic transaminase and alkaline phosphatase. KMBC is the second established cell line that originated from a human extrahepatic bile duct carcinoma in the world literature, and it will be applicable to various experiments.