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Placental‐like alkaline phosphatase and DNA flow cytometry in spermatocytic seminoma
Author(s) -
Dekker Inge,
Rozeboom Thea,
Delemarre Jan,
Dam Anke,
Oosterhuis Jan Wolter
Publication year - 1992
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19920215)69:4<993::aid-cncr2820690427>3.0.co;2-8
Subject(s) - alkaline phosphatase , seminoma , flow cytometry , medicine , placental alkaline phosphatase , pathology , biology , immunology , biochemistry , enzyme , chemotherapy
Immunohistochemical analysis was done on 7 testicular tumors classified as spermatocytic seminoma (SS) and 25 classic seminomas. Except for a few scattered cells, the spermatocytic seminomas were negative for placental‐like alkaline phosphatase (PLAP); the classic seminomas were all positive for this enzyme. The SS also were negative for alpha‐fetoprotein (AFP), beta‐human chorionic gonadotropin (hCG), and leukocyte common antigen (LCA). The ploidy of the seven tumors of SS was as follows: two, diploid; two, near‐diploid; one, tetraploid; one, aneuploid; and one, uninterpretable. The essentially negative staining of SS for PLAP was strikingly different from the pattern in classic seminoma. Thus, staining for this enzyme is useful for making the differential diagnosis between classic seminoma and SS. To differentiate between malignant lymphoma and SS, staining for leukocyte common antigen is helpful. Cancer 1992; 69:993–996.