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Distribution and therapeutic effect of intraarterially transferred tumor‐infiltrating lymphocytes in hepatic malignancies. A preliminary report
Author(s) -
Takayama Tadatoshi,
Makuuchi Masatoshi,
Sekine Teruaki,
Terui Shoji,
Shiraiwa Hiroshi,
Kosuge Tomoo,
Yamazaki Susumu,
Hasegawa Hiroshi,
Suzuki Kazuyoshi,
Yamagata Motoo,
Fujii Masashi,
Tanaka Takashi,
Kakizoe Tadao
Publication year - 1991
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19911201)68:11<2391::aid-cncr2820681110>3.0.co;2-7
Subject(s) - medicine , chemoimmunotherapy , immunotherapy , in vivo , tumor infiltrating lymphocytes , scintigraphy , cancer research , monoclonal antibody , pathology , immunology , antibody , cancer , biology , microbiology and biotechnology
Indium‐111–labeled tumor‐infiltrating lymphocytes ( 111 In‐TIL) were transferred as an intrahepatic arterial bolus to determine their in vivo distribution in patients with hepatic malignancies. In the in vitro culture system, TIL were expanded upon simultaneous stimulation by recombinant interleukin‐2 (rIL‐2) and immobilized anti‐CD3 monoclonal antibody. This double activation led not only to a larger cell yield, but also to a significantly more dominant subpopulation with CD4 + phenotype than occurred with activation by rIL‐2 alone. Accumulations of 111 In‐TIL in the liver were identified by scintigraphy in all of three patients, corresponding to the tumor localization by computed tomography. Such accumulation had persisted for at least 48 hours after infusion. After intraarterial chemoimmunotherapy that included TIL, two of three patients achieved a partial therapeutic response. The authors conclude that their method of culture and transfer can facilitate the accumulation of TIL at tumor sites, which may augment the antitumor effects of adoptive immunotherapy. Cancer 68:2391–2396, 1991.