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Potential of human lymph node cells for antitumor activity mediated by interferon gamma
Author(s) -
Okadome Masao,
Saito Toshiaki,
Tsukamoto Naoki,
Sano Masatoshi,
Kamura Toshiharu,
Nakano Hitoo
Publication year - 1991
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19911201)68:11<2378::aid-cncr2820681108>3.0.co;2-2
Subject(s) - medicine , clonogenic assay , concanavalin a , hela , lymph node , interferon gamma , tumor necrosis factor alpha , cytokine , cancer research , stimulation , cell culture , lymph , interferon , immunology , pathology , biology , in vitro , biochemistry , genetics
The soluble antitumor activity of regional lymph node cells obtained from patients with cervical cancer was investigated by using a human tumor clonogenic assay (HTCA). A significant antiproliferative activity of the lymph node cells (LNCs) against a cervical cancer cell line, HeLa cells, was demonstrated by stimulation with either phytohemagglutinin (PHA) or concanavalin A (Con A), but not with interleukin‐2 (IL‐2). This antiproliferative activity of LNC was found in nonadherent cells, possibly T‐cells. By using neutralizing antibody experiments, this activity was found to be attributed to interferon gamma (IFN γ), but not to tumor necrosis factor (TNF), although both cytokines were produced from LNC. These results indicate that human LNC was able to exert an antiproliferative activity mediated through the cytokines by appropriate stimulation. Cancer 68:2378–2383, 1991.