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Basic fibroblast growth factor and somatomedin C in human medulloepithelioma
Author(s) -
Shiurba Robert A.,
Buffinger Nicholas S.,
Spencer E. Martin,
Urich Henry
Publication year - 1991
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19910815)68:4<798::aid-cncr2820680423>3.0.co;2-c
Subject(s) - neuroepithelial cell , growth factor , immunostaining , basic fibroblast growth factor , fibroblast growth factor , biology , immunohistochemistry , somatomedin , autocrine signalling , population , endocrinology , pathology , medicine , immunology , microbiology and biotechnology , cell culture , stem cell , biochemistry , genetics , receptor , environmental health , neural stem cell
Two published cases of medulloepithelioma, a rare malignant pediatric brain tumor composed of a mixture of primitive neuroepithelium and its differentiated neuronal and glial descendants, were examined by immunohistochemical staining for the presence of growth factors. From a panel of antibodies, those identifying basic fibroblast growth factor and insulin‐like growth factor I, formerly known as somatomedin C, were strongly immunoreactive within the neuroepithelial cell population of the tumors. Immunoblots of purified recombinant basic fibroblast growth factor and insulin‐like growth factor I showed antibody specificity without cross‐reactivity. In controls, immunostaining of tissue sections was abolished by preabsorption of primary antibodies with the appropriate growth factor polypeptide antigen. Preabsorption with inappropriate growth factor did not reduce the intensity or alter the distribution of staining. The congruent histologic patterns of immunoreactivities suggest that more than one type of growth factor may be produced by the neuroepithelial component of medulloepithelioma. These growth factors may stimulate proliferation and differentiation of tumor cells by autocrine molecular mechanisms.