Premium
The importance of surface immunoglobulin, mouse rosettes, and cd5 in the immunophenotyping of chronic lymphocytic leukemia and reactive lymphocytosis
Author(s) -
Batata Al,
Shen Bo
Publication year - 1991
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19910715)68:2<355::aid-cncr2820680225>3.0.co;2-7
Subject(s) - immunophenotyping , lymphocytosis , chronic lymphocytic leukemia , rosette (schizont appearance) , cd5 , surface immunoglobulin , medicine , rosette formation , antibody , pathology , immunology , leukemia , b cell , flow cytometry
Peripheral blood from 167 B‐chronic lymphocytic leukemia (B‐CLL) and 119 reactive lymphocytosis (RLC) patients were analyzed to evaluate the immunophenotypic diagnostic value of mouse rosettes (M‐rosette), and weak expression of monoclonal surface immunoglobulin (SIg). In B‐CLL, 145 cases were M‐rosette+ (86.83%), 135 surface immunoglobulin (SIg)+ (80.84%), and 117 M‐rosette+ SIg+ (70.06%). Of 32 SIg– cases, 28 were M‐rosette+; and of 22 M‐rosette–cases, 18 were SIg+. By combining results of the two assays and accepting positivity of either one or both as sufficient for diagnosis, B‐CLL was diagnosed in 163 cases (97.60%). CD5 was performed in 49 cases of the 167 with paired data for SIg and M‐rosettes. By combining the results of the three assays and accepting positivity of any two or all three as sufficient for diagnosis, all 49 cases (100%) were diagnosed. Correlation analysis showed no significant association between M‐rosette, SIg, and CD5 expression. The results demonstrate the independent expression of the three markers, and their complementary role in immunophenotyping B‐CLL. In RLC, all 119 cases were T‐lineage and SIg–, and 115 were M‐rosette–, indicating the role of the two markers in differentiating B‐CLL from RLC. Three of the four M‐rosette+ T‐RLC were subsequently diagnosed as B‐CLL, suggesting the necessity of follow‐up of such cases.