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Multi‐parameter flow cytometric quantitation of the expression of the tumor‐associated antigen SM3 in normal and neoplastic ovarian tissues. A comparison with HMFG1 and HMFG2
Author(s) -
Van Dam Peter A.,
Jobling Thomas W.,
Shepherd John H.,
Lowe David G.,
Watson James V.,
Chard Tim
Publication year - 1991
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19910701)68:1<169::aid-cncr2820680130>3.0.co;2-r
Subject(s) - monoclonal antibody , immunohistochemistry , antigen , flow cytometry , epitope , pathology , mucin , antibody , muc1 , medicine , ovarian cancer , ovarian tumor , cancer research , microbiology and biotechnology , biology , cancer , immunology
SM3 is a monoclonal antibody that reacts with a peptide epitope in the core protein of polymorphic epithelial mucin. Multi‐parameter flow cytometry was used to characterize the expression of SM3 and compare it with two related tumorassociated antigens, HMFG1 and HMFG2, in cell suspensions of 44 malignant ovarian tumors, 15 benign ovarian tumors, and 16 normal ovaries. Tumorassociated antigen expression was significantly higher in malignant ovarian neoplasms than in benign neoplasms ( P less than 0.001 for all three antigens). SM3 was expressed more specifically in malignant than benign tumors but had a lower affinity than HMFG1 and HMFG2. Multi‐parameter flow cytometric evaluation of a panel of monoclonal antibodies can be used to help in choosing the best antibody for immunohistochemistry, imaging, and eventually treatment of ovarian tumors.