Premium
Nuclear DNA profiles in primary melanomas and their metastases
Author(s) -
Rode J.,
Williams R. A.,
Charlton I. G.,
Dhillon A. P.,
Moss Eileen
Publication year - 1991
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19910501)67:9<2333::aid-cncr2820670920>3.0.co;2-f
Subject(s) - ploidy , melanoma , primary tumor , metastasis , pathology , nuclear dna , feulgen stain , medicine , primary (astronomy) , dna , cancer , biology , cancer research , staining , genetics , physics , astronomy , gene , mitochondrial dna
DNA‐aneuploid primary melanomas are said to show a greater propensity to metastasize than those without an aneuploid profile. A higher aneuploid rate has also been reported in metastatic melanomas than in primary lesions. The ploidy profile was determined of 26 primary melanomas and their first subsequent secondaries on Feulgen‐stained sections from routinely processed material using a computerized video image‐analysis system. In 18 of 26 cases, both primary and secondary tumor showed similar corresponding DNA patterns. These were aneuploid in 13 of 18 of the cases. A hyperdiploid pattern was present in one case in both the primary and its metastasis, and a diploid pattern was seen in four cases. There were only eight cases in which a disparity between the nuclear ploidy profile in the primary and secondary tumor was evident. Although the results suggest that ploidy studies are not useful for predicting the metastatic potential of a primary melanoma, the maintenance of similar DNA profiles in the metastases in most of these cases was interesting in regard to tumor biology.