Premium
Flow cytometric determination of breast tumor heterogeneity
Author(s) -
Fuhr Joseph E.,
Frye Ashley,
Kattine Anthony A.,
Van Meter Stuart
Publication year - 1991
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19910301)67:5<1401::aid-cncr2820670521>3.0.co;2-4
Subject(s) - aneuploidy , flow cytometry , dna , pathology , population , medicine , mammary gland , breast cancer , microbiology and biotechnology , biology , cancer , genetics , chromosome , environmental health , gene
Flow cytometric analysis was done on the DNA content of nuclei obtained from different sites of small breast tumors. Although specimens for analysis were obtained within a few millimeters of each other, dramatic differences were occasionally observed in the DNA histograms. In a limited study involving 141 consecutive breast specimens submitted for flow cytometry, 52% (74) were found to have at least one DNA aneuploid population. In 18% of DNA aneuploid tumors, one or more specimens from areas grossly identified as tumor had no DNA aneuploid population. Because of the proposed correlation of aneuploidy with a poorer prognosis and possible responsiveness to chemotherapy, multiple sites should be assayed when flow cytometric DNA analysis is done.