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Amplification of oncogenes in mammary carcinoma shown by fine‐needle biopsy
Author(s) -
Lönn Ulf,
Lönn Sigrid,
Nylen Urban,
Winblad Gerard,
Stenkvist Björn
Publication year - 1991
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19910301)67:5<1396::aid-cncr2820670520>3.0.co;2-n
Subject(s) - cancer , medicine , gene duplication , biopsy , oncogene , mammary carcinoma , mammary gland , pathology , cancer cell , cancer research , southern blot , sampling (signal processing) , carcinoma , gene , oncology , breast cancer , biology , genetics , cell cycle , filter (signal processing) , computer science , computer vision
A procedure that measures the amplification of oncogenes in human cancer cells is described. The cells were obtained by fine‐needle biopsy to allow repeated sampling from individual metastases. A drawback was the low number of cells obtained, but this could be overcome by using a slot–blot hybridization technique to measure gene amplification. Two patients with mammary cancer (primary tumors or metastases), analyzed for the levels of amplification of the oncogene erb ‐B2, are described in detail. This technique is suitable for analyzing alterations occurring during cancer progression and for identifying subgroups of mammary cancer with different characteristics.