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Flow cytometric dna analysis of hepatocellular carcinoma
Author(s) -
Fujimoto Jiro,
Okamoto Eizo,
Yamanaka Naoki,
Toyosaka Akihiro,
Mitsunobu Masao
Publication year - 1991
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19910215)67:4<939::aid-cncr2820670414>3.0.co;2-j
Subject(s) - nuclear dna , hepatocellular carcinoma , aneuploidy , medicine , flow cytometry , dna , pathology , metastasis , gastroenterology , ploidy , cancer , biology , immunology , gene , mitochondrial dna , genetics , chromosome
Prognostic value of nuclear DNA content was studied retrospectively using flow cytometry in 203 cases of resected hepatocellular carcinoma. The occurrence of DNA aneuploidy, which was detected in 50% of patients, correlated significantly with tumor size and the presence of vascular invasion or intrahepatic metastasis. Overall, patients with DNA aneuploid tumors had a significantly worse prognosis than those with DNA diploid tumors ( P < 0.001) and, also in subdivided groups by tumor size ( P < 0.01). Among DNA aneuploid patients, the survival times were significantly shorter for patients with a low DNA index (< 1.5) than for those with a high DNA index (≥1.5) ( P < 0.05). In a Cox multivariate analysis, nuclear DNA content provided significant prognostic value ( P = 0.008), as did vascular invasion ( P = 0.001) and intrahepatic metastasis ( P = 0.005). These results indicated that nuclear DNA content has an important prognostic value in hepatocellular carcinoma.