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Molecular phenotype of a pediatric small round cell tumor
Author(s) -
Bendit Israel,
Johnston Jean,
Valderrama Elsa,
Waber Pamela,
Nisen Perry D.
Publication year - 1990
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19901001)66:7<1534::aid-cncr2820660717>3.0.co;2-g
Subject(s) - wilms' tumor , neoplasm , southern blot , pathology , rna , gene , biology , oncogene , dna , northern blot , phenotype , microbiology and biotechnology , medicine , genetics , cell cycle
Molecular probes were used to characterize an unusual small round cell abdominal tumor arising from the fallopian tube of a 15‐year‐old girl. DNA and RNA extracted from the tumor and adjacent normal tissue was subjected to Southern and Northern blot analysis using a variety of different probes. N‐ myc oncogene RNA was greatly expressed in the tumor, but was not expressed in normal tissue or amplified in chromosomal DNA. Insulin‐like growth factor II RNA was similarly overexpressed in the neoplasm, but not in normal tissue. While histopathologic studies could not distinguish between a neuroectodermal neoplasm and Wilms' tumor, electron microscopy and the pattern of gene expression was most consistent with Wilms' tumor.