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Flow cytometric DNA analysis after immunoselection of bladder tumor cells with monoclonal antibody DU83.21
Author(s) -
Wright George L.,
Alexander Jeannine P.,
Konchuba Alice M.,
Schellhammer Paul F.,
Schlossberg Steven M.
Publication year - 1990
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19900915)66:6<1242::aid-cncr2820660626>3.0.co;2-y
Subject(s) - monoclonal antibody , propidium iodide , stain , microbiology and biotechnology , flow cytometry , pathology , medicine , cell culture , carcinoma , cancer cell , antibody , bladder cancer , cancer research , cancer , biology , immunology , staining , apoptosis , programmed cell death , biochemistry , genetics
Adequate preservation of neoplastic cells and the elimination of interference by inflammatory cells in measuring tumor cell DNA content represent two important objectives necessary for accurate flow cytometric analysis of bladder carcinomas. An experimental model consisting of a mixture of cultured bladder carcinoma cells (T24) and human buffy‐coat (BC) cells was used to evaluate various preservatives and an anti‐bladder carcinoma monoclonal antibody (MoAb), DU83.21, for separating inflammatory cells (BC cells) from T24 cells. A final concentration of 25% ethanol was found to be the most effective preservative of several tested. After incubation with the MoAb DU83.21 and propidium iodide (DNA stain), the T24 cells could be separated from the BC cells, permitting accurate DNA analysis of the tumor cells. Application of this system to specimens from bladder cancer patients enhanced the detection and DNA analysis of the tumor cell populations.