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Association of point mutation in c‐Ki‐ ras oncogene in lung adenocarcinoma with particular reference to cytologic subtypes
Author(s) -
Kobayashi Toshiaki,
Tsuda Hitoshi,
Noguchi Masayuki,
Hirohashi Setsuo,
Shimosato Yukio,
Goya Tomoyuki,
Hayata Yoshihiro
Publication year - 1990
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19900715)66:2<289::aid-cncr2820660216>3.0.co;2-6
Subject(s) - point mutation , mutation , adenocarcinoma , medicine , pathology , polymerase chain reaction , lung cancer , microbiology and biotechnology , cancer research , cancer , gene , biology , genetics
The correlation between clinicopathologic findings and point mutation in codon 12 of c‐Ki‐ ras gene was examined in primary lung adenocarcinomas using polymerase chain reaction and oligonucleotide hybridization techniques. The mutation was detected in ten of 67 cases (15%). Microscopically, mutation‐positive cases revealed a tendency to be well differentiated ( P < 0.01). Especially, the incidence of the mutation‐positive cases was significantly higher in goblet cell type (three of four) than in other types (five of 56) ( P < 0.001). None of 21 cases of pure Clara cell type showed the mutation ( P < 0.05). The mutation was detected frequently in tumors with no lymph node metastasis ( P < 0.05), with larger tumor size ( P = 0.01), and T2 cases ( P < 0.01). Cigarette smoking was not always a contributing factor for mutation. This study revealed that the point mutation of c‐Ki‐ ras codon 12 in lung adenocarcinoma has been associated with the cytologic subtype.