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Human papillomavirus type 16/18 in uterine cervical adenocarcinoma in situ and adenocarcinoma a study by in situ hybridization with biotinylated DNA probes
Author(s) -
Nielsen Anette Lynge
Publication year - 1990
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19900601)65:11<2588::aid-cncr2820651132>3.0.co;2-s
Subject(s) - in situ hybridization , in situ , hybridization probe , pathology , medicine , biotinylation , adenosquamous carcinoma , human papillomavirus , adenocarcinoma , southern blot , microbiology and biotechnology , dna–dna hybridization , dna , biology , chemistry , cancer , gene , genetics , gene expression , organic chemistry
The distribution of human papillomavirus (HPV) DNA type 16/18 in the paraffin sections of 11 adenocarcinomas (AC), 4 adenocarcinomas in situ (AIS), and 2 adenosquamous carcinomas (AC/SCC) of the cervix was examined by in situ hybridization (ISH) with biotinylated DNA probes (B‐probes). Four AC, four AIS, and one AC/SCC were positive. These results show that B‐probes are an acceptable alternative to both ISH with radiolabeled probes and Southern blot hybridization (SBH), which are difficult to apply in routine pathologic laboratories. Cervical tissues infiltrated with endometrial AC were negative for HPV DNA 16/18, and it is suggested that the presence of HPV DNA 16/18 in uterine AC may be helpful in discriminating cervical AC from endometrial AC.