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A cell culture, chromosomal and quantitative dna analysis of a metastatic epithelioid sarcoma deletion 1p, a possible primary chromosomal abnormality in epithelioid sarcoma
Author(s) -
Stenman GÖRan,
Kindblom LarsGunnar,
Willems Jan,
Angervall Lennart
Publication year - 1990
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19900501)65:9<2006::aid-cncr2820650921>3.0.co;2-q
Subject(s) - epithelioid sarcoma , medicine , pathology , abnormality , sarcoma , epithelioid cell , chromosomal abnormality , karyotype , chromosome , biology , immunohistochemistry , genetics , gene , psychiatry
The chromosomal banding pattern and the in vitro growth characteristics of a metastatic epithelioid sarcoma are described. The cultured tumor cells retained growth characteristics as well as ultrastructural and immunohistochemical properties similar to the cells of the primary tumor. Cytogenetic analysis revealed a modal range in the diploid‐hypodiploid region, a finding which was corroborated by quantitative DNA determinations of both the primary tumor and a lymph node metastasis. Fourteen different marker chromosomes were identified. The most frequent clonal rearrangement was a 1p‐ marker resulting from a short arm terminal deletion, i.e. , del (1) (p21‐22). A similar 1p‐ marker has previously been observed in an established epithelioid sarcoma cell line. The finding of an apparently identical 1p‐ marker in two of two analyzed epithelioid sarcomas suggests that this rearrangement may be a primary cytogenetic abnormality in epithelioid sarcoma. An elevated ras p21 expression was demonstrated using immunohistochemical methods. The possible involvement of the N‐ ras gene and/or a tumor suppressor in the 1p deletion is considered.