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The demonstration of human papillomavirus 16 genomes in the nuclei of genital cancers using two different methods of in situ hybridization
Author(s) -
GrußendorfConen ElkeIngrid,
Cremer Sabine
Publication year - 1990
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19900115)65:2<238::aid-cncr2820650210>3.0.co;2-7
Subject(s) - in situ hybridization , in situ , biotinylation , hybridization probe , dna–dna hybridization , nucleic acid thermodynamics , human papillomavirus , biopsy , dna , carcinoma in situ , pathology , medicine , biology , microbiology and biotechnology , carcinoma , gene , chemistry , genetics , base sequence , gene expression , organic chemistry
Biopsy specimens of 16 invasive genital cancers (two vulval carcinomas, two carcinomas of the vagina, and 12 cervix carcinomas) were examined for the presence and distribution of human papillomavirus (HPV) DNA by in situ hybridization with 3 H‐labeled and biotinylated DNA probes of HPV 6, HPV 11, and HPV 16. None of the tumors reacted with HPV 6 or HPV 11. Using in situ hybridization with 3 H‐labeled DNA probes nine of the 16 cancers gave positive results with HPV 16. Only three of the nine were positive for HPV 16 by in situ hybridization with biotinylated probes. Currently, the method of in situ hybridization with commercial biotinylated probes is less sensitive than in situ hybridization with 3 H‐labeled HPV DNA.