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Hormone receptor status in human endometrial adenocarcinoma
Author(s) -
De Cicco Nardone Fiorenzo,
Benedetto Maria Teresa,
Rossiello Francesco,
Bongiorno Maurizio,
Iacobelli Stefano,
Mancuso Salvatore,
Dell'Acqua Sergio
Publication year - 1989
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19891215)64:12<2572::aid-cncr2820641227>3.0.co;2-n
Subject(s) - receptor , endocrinology , medicine , dissociation constant , endometrium , cytosol , hormone receptor , sex hormone receptor , hormone , chemistry , estrogen receptor , cancer , enzyme , biochemistry , breast cancer
Steroid receptor levels were determined in 196 samples of endometrial adenocarcinoma: cytosol estradiol receptors (ERc) were measured in 171 samples, cytosol progesterone receptors (PRc) in all samples; nuclear estradiol receptors (ERn) and nuclear progesterone receptors (PRn) in 68 samples; total estradiol receptors (ERt = ERc plus ERn) and total progesterone receptors (PRt = PRc plus PRn) were measured in 68 samples. The ERc levels were 88.2 ± 8.9 (mean ± SEM) and ERn were 94.4 ± 15.6 fmol/mg protein; PRc levels were 197.9 ± 25.9 and PRn 178.3 ± 55.9 fmol/mg protein. The ERt levels were 162.6 ± 23.2 and PRt 249.8 ± 75.7 fmol/mg protein. The presence of PRc was related to the ERc levels according to the cut‐off used. Estradiol receptors (ER) and progesterone receptors (PR) were present in the cytoplasmic and nuclear fractions in 60.2% and 36.8% of cases, respectively. The simultaneous presence of both ERt and PRt was observed only in 27.9% of cases. In the normal endometrium ERc and PRc were negatively correlated (r = −0.525, P < 0.005), whereas in endometrial adenocarcinoma the correlation was positive (r=0.491, P < 0.001). In contrast with the normal endometrium the correlation between ERc and ERn was positive (r=0.582, P < 0.001) in tumor tissue. In neoplastic tissue Scatchard analysis showed a single class of specific ERc sites with a dissociation constant (Kd) of 1.39 ± 0.8 × 10 −9 mol/I, one tenth of that found in the normal premenopausal endometrium. Qualitative and quantitative analysis of the receptor status showed that in 30% to 40% of cases studied the behavior of the neoplastic cell was similar to that found in the normal endometrial cell. In a 4‐year follow‐up of patients affected by endometrial adenocarcinoma there is better survival in the groups of patients with a simultaneous presence of ERt and PRt than in the group with their absence. Cancer 64:2572–2578, 1989.