Premium
Correlation of tumor necrosis factor and prostaglandin E 2 production of monocytes in bladder cancer patients
Author(s) -
Ikemoto Shinichi,
Kishimoto Taketoshi,
Nishio Shoichi,
Wada Seiji,
Maekawa Masanobu
Publication year - 1989
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19891115)64:10<2076::aid-cncr2820641018>3.0.co;2-x
Subject(s) - medicine , prostaglandin e2 , tumor necrosis factor alpha , cancer , tumor necrosis factor α , bladder cancer , oncology , cancer research
Patients with advanced malignant neoplasms have a variety of abnormal monocyte and lymphocyte functions. The authors examined tumor necrosis factor (TNF) and prostaglandin E 2 (PGE 2 ) production of monocytes in 48 patients with bladder cancer and 16 control subjects. Monocytes were isolated from peripheral blood mononuclear cells by adherence to plastic tissue. They were cultured with lipopolysac‐charide for 24 hours, and the culture supernatant was obtained. The TNF was measured by enzyme immunoassay using anti‐recombinant human TNF antibody, and PGE 2 was measured by radioimmunoassay. As a result, in high‐stage bladder cancer patients, there was a significant inverse correlation between TNF and PGE 2 production of monocytes. However, there was no significant correlation in control subjects and low stage patients. Accordingly, some patients with high‐stage bladder cancer had higher TNF production but lower PGE 2 production of monocytes, and vice versa .