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Tumor acquisition, propagation, and preservation. The culture of human colorectal cancer
Author(s) -
Lewko Walter M.,
Ladd Patricia,
Hubbard Diane,
He YunJu,
Vaghmar Rupa,
Husseini Salah,
Chang Lee,
Moore Marthanna,
Thurman Gary B.,
Oldham Robert K.
Publication year - 1989
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19891015)64:8<1600::aid-cncr2820640808>3.0.co;2-s
Subject(s) - cell culture , colorectal cancer , medicine , pathology , biopsy , tissue culture , cancer , cell , cancer research , biology , in vitro , genetics
Fourteen new colorectal cancer cell lines were developed as part of a tumor acquisition, propagation, and preservation program for biotherapy. Fifty‐six specimens were received. Nine cell lines were generated from biopsies; seven of these cell lines were from metastatic lesions. Five additional cell lines were developed from xenografts grown in nude mice. Biopsies that produced three of these xenografts gave rise to parallel culture cell lines. Biopsy‐derived and xenograft‐derived cell lines from the same tumor behaved similarly in culture and exhibited similar markers when assessed immunohistochemically. Collagen substrate was beneficial in the primary culture of 50% of the specimens tested. Collagen was required for the successful propagation of two cell lines.