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The establishment of epstein‐barr virus nuclear antigen‐positive (SP‐50B) and epstein‐barr virus nuclear antigen‐negative (SP‐53) cell lines with t(11;14)(q13;q32) chromosome abnormality from an intermediate lymphocytic lymphoma
Author(s) -
Daibata Masanori,
Kubonishi Ichiro,
Eguchi Taisuke,
Yano Shoki,
Ohtsuki Yuji,
Miyoshi Isao
Publication year - 1989
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19890915)64:6<1248::aid-cncr2820640614>3.0.co;2-5
Subject(s) - lymphoma , virus , epstein–barr virus , biology , antigen , virology , cell culture , chromosome , microbiology and biotechnology , immunology , genetics , gene
Two lymphoma cell lines, SP‐50B and SP‐53, were established from peripheral blood of a 58‐year‐old woman with leukemic conversion of intermediate lymphocytic lymphoma. These cell lines grew in suspension with or without forming clumps of cells. SP‐50B was morphologically similar to the common Epstein‐Barr (EB) virus‐transformed lymphoblastoid cell lines and was positive for EB virus nuclear antigen (EBNA), whereas SP‐53 closely resembled the patient's lymphoma cells and was negative for EBNA. Both cell lines expressed the same phenotypic markers as original lymphoma cells (CpIg+, SmIg+, OKIaI+, Leu12+) and possessed t(11;14)(q13;q32) chromosome translocation. These results indicate that although morphologically different, SP‐50B and SP‐53 were both derived from patient's lymphoma cells. The long‐term cultivation of EBNA‐positive and EBNA‐negative B‐cell lymphoma lines from a single donor has not been previously reported. These cell lines would provide useful tools for studying the oncogenic role of EB virus and bcl ‐1 oncogene that is located on chromosome 11q13.