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B‐cell lymphoma marking only with anti‐epithelial. Membrane antigen
Author(s) -
Mark Abraham S.,
Mangkornkanok Marayart
Publication year - 1989
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19890601)63:11<2152::aid-cncr2820631114>3.0.co;2-q
Subject(s) - lymphoma , epitope , wheat germ agglutinin , antigen , agglutinin , galactosamine , antibody , soybean agglutinin , peanut agglutinin , b cell lymphoma , lectin , medicine , microbiology and biotechnology , biochemistry , biology , immunology , galactose
A large cell malignancy identified as a B‐lymphoma only by virtue of μ and kappa chain gene rearrangement additionally displayed profuse plasmalemma marking with anti‐epithelial membrane antigen (EMA). Affinity absorption of the antibody with N‐acetyl‐galactosamine but not with N‐acetylglucosamine or d‐galactose effectively removed the lymphoma reactive antibodies. Soy bean agglutinin but not wheat germ agglutinin demonstrated lectin activity towards an epitope on the lymphoma membrane. Finally, digestion of cryostat sections of the lymphoma with N‐acetylgalactosaminidase effectively removed the sugar epitope involved in EMA marking. Such data taken together would tend to indicate a membrane positioned complex carbohydrate with galactosamine dominancy. By analogy to the purported “late differentiation antigen secretory function” of EMA this lymphoma may be close to plasma cell stage.