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Spontaneous secretion of tumor necrosis factor‐beta by human myeloma cell lines
Author(s) -
Bataille Régis,
Klein Bernard,
Jourdan Michel,
François Rossi Jean,
Durie Brian G. M.
Publication year - 1989
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19890301)63:5<877::aid-cncr2820630514>3.0.co;2-6
Subject(s) - tumor necrosis factor alpha , medicine , secretion , cytokine , multiple myeloma , bone resorption , osteoclast , endocrinology , cancer research , receptor
Abstract Abnormal bone remodelling in the vicinity of tumor cells is a common, early feature of multiple myeloma, characterized by excessive bone resorption which is mediated by local soluble factors called osteoclast activating factors (OAF). Since interleukin‐1 (IL‐1) and tumor necrosis factors (TNF) are potent, synergistic OAF produced by cells of the B‐cell lineage, the authors investigated the spontaneous secretion of these cytokines by 11 human myeloma cell lines (HMCL). No HMCL secreted either IL‐1, the most powerful OAF, or contra IL‐1. In contrast, all of the lymphoblastoid cell lines assayed produced significant IL‐1 activity. Ten of the 11 HMCL secreted a significant TNF activity. This was completely eliminated by an anti‐TNFβ monoclonal antibody but not affected by an anti‐TNFα antiserum. The data from this study suggest that TNFβ is involved in myeloma bone resorption, but not IL‐1, which is, however, known as one of the most potent OAF.