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Characterization of pyruvate kinase from human rhabdomyosarcoma in relation to immunohistochemical and morphological criteria
Author(s) -
Staal Gerard E. J.,
Rijksen Gert,
Van Oirschot Brigit A.,
Roholl Paul J. M.
Publication year - 1989
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19890201)63:3<479::aid-cncr2820630315>3.0.co;2-m
Subject(s) - desmin , immunohistochemistry , pyruvate kinase , rhabdomyosarcoma , myoglobin , pathology , isozyme , biology , sarcoma , medicine , enzyme , biochemistry , vimentin , glycolysis
We have compared the pyruvate kinase (PK) isoenzyme pattern of three rhabdomyosarcomas with foetal skeletal muscle tissue of 19 and 23 weeks of gestation, together with adult muscle in relation to immunohistochemical and morphological criteria. In foetal tissue of 19 weeks of gestation a focal immunopositivity for desmin and myoglobin was observed, whereas in tissue of 23 weeks an overall positivity for these proteins was present. Two of the three neoplasms were poorly differentiated and of the alveolar subtype. They were desmin immunoreactive. Some large spindle‐shaped cells expressed myoglobin. The third one was more differentiated in microscopic characteristics and all cells showed immunoreactivity for desmin and myoglobin. In the foetal tissues five forms of pyruvate kinase isoenzymes were present with K 2 M 2 as the predominant form. In adult muscle tissue only M 4 was present. The tumors were characterized by a profound shift to the K‐type, whereas the M 4 ‐type was not expressed at all. A difference in isoenzyme composition of pyruvate kinase was found between the morphologically less differentiated tumors and the more differentiated tumor; in the latter more M‐subunits were expressed.