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Fulminant clonal expansion of large granular lymphocytes: Characterization of their morphology, phenotype, genotype, and function
Author(s) -
Ohno Tatsuharu,
Kanoh Tadashi,
Arita Yuu,
Fujii Iroshi,
Kuribayashi Kagemasa,
Masuda Tohru,
Horiguchi Yuji,
Taniwaki Masashi,
Nosaka Tetsuya,
Hatanaka Masakazu,
Uchino Haruto
Publication year - 1988
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19881101)62:9<1918::aid-cncr2820620909>3.0.co;2-8
Subject(s) - cd16 , immunology , cd3 , cd8 , biology , antigen , natural killer cell , immune system , cytotoxic t cell , microbiology and biotechnology , in vitro , genetics
A 39‐year‐old woman exhibited abrupt malignant transformation of the large granular lymphocytes (LGL) after a chronic course of T γ ‐lymphoproliferative disease (T γ 7‐LPD). The T γ ‐lymphocytes were CD2+, CD3‐, CD8‐, CD16+, Leu7‐, and Leul9+ with morphologic characteristics of LGL. Newly appearing LGL were much larger and had more prominent azurophilic granules. Although fundamentally they had the same phenotype as the LGL in chronic stage, they showed increased la‐like antigen and decreased CD16 antigen expressions. Immunoglobulin (Ig) G‐kappa type monoclonal component was detected in the patient's serum. The LGL showed a germ‐line configuration for T‐cell receptor (TCR) beta and gamma chain genes, whereas the clonal chromosomal abnormalities indicated the neoplastic nature of the LGL. The LGL exhibited competent natural killer (NK), interleukin 2 (IL2) activated killer (AK), and antibody‐dependent cell‐mediated cytotoxicity (ADCC) activities. The LGL may have derived from NK cells at their mature stage with prethymic phenotype and may have influenced the homeostasis of the patient's humoral immune response.