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Demonstration of chromogranin a in human neuroendocrine cell lines by immunohistology and immunoassay
Author(s) -
Deftos L. J.,
Linnoila R. I.,
Carney D. N.,
Burton D. W.,
Leong S. S.,
O'Connor D. T.,
Murray S. S.,
Gazdar A. F.
Publication year - 1988
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19880701)62:1<92::aid-cncr2820620117>3.0.co;2-t
Subject(s) - chromogranin a , radioimmunoassay , cell culture , calcitonin , medicine , neuroendocrine cell , pathology , neuroendocrine differentiation , cell , cancer research , immunohistochemistry , biology , cancer , genetics , prostate cancer
We have used immunohistology and radioimmunoassay procedures to study Chromogranin A (CgA) in human neuroendocrine tumor cell lines, especially small cell lung cancers (SCLC). By immunohistology, CgA could be detected in 11 of 18 classical SCLC cell lines, in a medullary thyroid carcinoma (MTC) cell line, and in only one of 13 variant‐ or non‐SCLC cell lines. By radioimmunoassay, CgA could be detected in the cells and culture media of all of the classical SCLC cell lines tested. Many of the classical SCLC cell lines also produced calcitonin (CT). These studies demonstrate that CgA production is a common feature of SCLC cell lines, especially those with neuroendocrine characteristics.