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Demonstration of intracytoplasmic immunoglobulin in non‐T‐acute lymphocytic leukemia cases of children with immunoelectron microscopic examination
Author(s) -
Mori Naoyoshi,
Oka Kuniyuki,
Hanada Takashi,
Iizuka Atsuo,
Kojima Mizu
Publication year - 1988
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19880601)61:11<2231::aid-cncr2820611117>3.0.co;2-w
Subject(s) - calla , immunoelectron microscopy , medicine , antibody , immunoglobulin light chain , chronic lymphocytic leukemia , pathology , antigen , acute lymphocytic leukemia , monoclonal antibody , cd20 , leukemia , cytoplasm , immunology , biology , lymphoblastic leukemia , biochemistry
Leukemic cells from 25 cases of non‐T‐acute lymphocytic leukemia (ALL) in children were investigated for intracellular immunoglobulin by the use of immunoelectron microscopic study (immuno‐EM). As a result, 12 cases were negative for both heavy and light chains of the immunoglobulin in the neoplastic cells. Of these, one case that was also negative for common ALL antigen (CALLA) was defined as null cell type. The remaining 11 cases were defined as common ALL type. Six cases were defined as pre‐B‐cell type because they were positive for cytoplasmic μ chain but negative for cytoplasmic light chains. Seven cases were defined as B‐cell type, all of which possessed λ light chains in the neoplastic cells. Immunohistologic studies using monoclonal antibodies showed that most non‐T‐ALL cases were positive for CALLA and B 4 , whereas about 50% of them were positive for B 1 . Our immuno‐EM study suggests that it is one of the effective ways of subclassifying non‐T‐ALL in children.