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An immunochemical and immunohistochemical study of S100 protein in renal cell carcinoma
Author(s) -
Takashi Munehisa,
Haimoto Hajime,
Murase Tatsuro,
Mitsuya Hideo,
Kato Kanefusa
Publication year - 1988
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19880301)61:5<889::aid-cncr2820610506>3.0.co;2-7
Subject(s) - s100 protein , immunohistochemistry , renal cell carcinoma , pathology , renal cortex , staining , alpha (finance) , medicine , carcinoma , kidney , construct validity , nursing , patient satisfaction
The authors localized S100 protein in renal tubules and renal cell carcinoma by immunohistochemical study and quantitative analysis by enzyme immunoassay. The α subunit of S100 protein (S100‐α) was localized in epithelial cells of proximal tubules, thin limbs of loops of Henle, collecting tubules, and a few of Bowman's capsules. The β subunit (S100‐β) was immunostained in the distal tubules, thick and thin loops of Henle, collecting tubules, and a few proximal tubules. In renal cell carcinoma, S100‐α was immunohistochemically demonstrated in 82% (41/50) of patients including sarcomatoid variants, whereas S100‐β was detected in 46% (23/50). Both the number of positively stained tumor cells and the staining intensity were greater in S100‐α than in S100‐β. Concentrations of S100‐α in the cortex were 80.3 ± 22.5 ng/mg protein (n = 7), whereas those of renal cell carcinoma were 387 ± 533 ng/mg protein (n = 19), i.e. , about five times higher. Concentrations of S100‐β in both normal kidney (1.96 ± 0.74 ng/mg protein) and renal cell carcinoma (2.05 ± 2.16 ng/mg protein) were much lower than those of S100‐α. The authors also localized S100‐α and S100‐β in tissues of other renal tumors and tumors arising from other organs. S100a o appears to be a useful immunohistochemical marker for renal cell carcinoma.