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Reed‐sternberg cells and their cell microenvironment in Hodgkin's disease with reference to macrophage‐histiocytes and interdigitating reticulum cells
Author(s) -
Carbone Antonino,
Manconi Riccardo,
Poletti Alessandro,
Sulfaro Sandro,
Menin Alessandro,
Tirelli Umberto,
Betta Piero G.,
Volpe Rachele
Publication year - 1987
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19871201)60:11<2662::aid-cncr2820601115>3.0.co;2-s
Subject(s) - nodular sclerosis , histiocyte , pathology , immunostaining , immunoperoxidase , antigen , lymph node , immunohistochemistry , monoclonal antibody , macrophage , lymphocyte , lymphoma , antibody , medicine , biology , microbiology and biotechnology , immunology , hodgkin lymphoma , biochemistry , in vitro
Fifty‐eight paraffin‐embedded lymph node biopsies from patients with Hodgkin's disease (36 nodular sclerosis, 14 mixed cellularity, five lymphocyte depletion, and three lymphocyte predominance) were immunostained with a panel of monoclonal (anti‐Leu‐MI, antileukocyte common antigen) and polyclonal (to lysozyme, alpha 1 ‐antitrypsin, alpha 1 ‐antichymotrypsin, and S‐100 protein) antibodies by using the avidin‐biotin immunoperoxidase technique. Both the immunostaining features of the Reed‐Sternberg (R‐S) cells and their variants, and the numbers of immunostained accompanying cells morphologically corresponding to macrophage‐histiocytes (M‐H) and to interdigitating reticulum cells (IRC) were analyzed. Variable numbers of R‐S cells and their variants were positive for Leu‐M1 in 83% of the cases, for alpha 1 ‐antitrypsin in 40%, for alpha 1 ‐antichymotrypsin in 30%, and for leukocyte common antigen in 3.4%; they were constantly negative for lysozyme and S‐100 protein. Whereas the average numbers of accompanying cells immunostained for Leu‐M1 were very low, the numbers of S‐100‐positive IRC were relatively high in all the Hodgkin's subtypes. The average numbers of M‐H were lower ( P < 0.1 for lysozyme; P < 0.001 for alpha 1 ‐antichymotrypsin) in the nodular sclerosis than in the other pooled subtypes. In the nodular sclerosis subtype, however, R‐S cells and their variants that stained positive for Leu‐M1 appeared to express more frequently the lineage markers of M‐H (alpha 1 ‐antitrypsin and/or alpha 1 ‐antichymotrypsin). These data appear to suggest that there is not an apparent qualitative correspondence between the immunostaining features of the cellular microenvironment composed of M‐H and IRC and the features of the R‐S cells.