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Detection of human papillomavirus‐6 in primary carcinoma of the urethra in men
Author(s) -
GrussendorfConen ElkeIngrid,
Deutz Friedrich Johannes,
De Villiers Ethel Michele
Publication year - 1987
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19871015)60:8<1832::aid-cncr2820600826>3.0.co;2-2
Subject(s) - immunoperoxidase , in situ hybridization , pathology , epithelioma , southern blot , carcinoma , human papillomavirus , carcinoma in situ , antigen , medicine , cancer , dna–dna hybridization , urethra , dna , biology , antibody , gene , monoclonal antibody , immunology , gene expression , anatomy , biochemistry , genetics
The authors report a case of primary urethral carcinoma which seems to be linked to human papillomavirus (HPV) infection. Southern blot hybridization of phosphorus 32 ( 32 P)‐labeled DNA extracted from the cancer tissue gave a positive reaction with HPV‐6. Specimens of the tumor material subjected to in situ hybridization with 3 H‐labeled HPV‐6 DNA showed numerous tumor cell clusters with clearly labeled nuclei. Using immunoperoxidase staining the authors found papillomavirus structural antigen within cell nuclei of the tumor tissue.