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Flow cytometric analysis of nephroblastomas and related neoplasms
Author(s) -
Schmidt Dietmar,
Wiedemann Bernd,
Keil Wolfgang,
Sprenger Ernst,
Harms Dieter
Publication year - 1986
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19861201)58:11<2494::aid-cncr2820581124>3.0.co;2-j
Subject(s) - pathology , wilms' tumor , flow cytometry , medicine , kidney , sarcoma , cytogenetics , oncology , biology , chromosome , immunology , genetics , gene
A total of 59 cases of nephroblastoma and related neoplasms were studied by flow cytometry of paraffin‐embedded tissue. According to clinical prognosis, cases were subdivided into three groups: Group 1 (low risk) consisted of congenital mesoblastic nephroma (n = 13) and cystic, partially differentiated nephroblastoma (n = 2). Group 2 (intermediate risk) comprised the various subtypes of „typical” nephroblastoma (n = 24) including cases of fetal rhabdomyomatous nephroblastoma (n = 4). In group 3 (high risk) there were cases of anaplastic nephroblastoma (n = 3), clear cell sarcoma of the kidney or „bone metastasizing renal tumor of childhood” (n = 7), and malignant rhabdoid tumor of the kidney (n = 6). The three clinically different groups of tumors also varied in the proportion of cases with aneuploid tumor DNA stemlines, in S‐phase fractions, and in proliferation indices (PI = S + G 2 + M). Group 1 was generally characterized by a small number of cases with aneuploid tumor DNA stemlines and low values for S‐phase fractions and PI, whereas Group 3 showed the largest number of cases with aneuploid tumor DNA stemlines and high values for S‐phase fractions and PI. Group 2 was in between. It is concluded that flow cytometry on paraffin‐embedded tissue from pediatric tumors may be a useful adjunct in determining prognosis, and that the subdivision of nephroblastomas and related neoplasms into three prognostically different groups is warranted.