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Determination of transferrin receptors on frozen sections of malignant B‐cell lymphomas by immunofluorescence with a monoclonal antibody
Author(s) -
Oudemans Pieter B.,
De La Rivière Guy Brutel,
Hart Guus A. M.,
van Heerde Peter,
Scholte Ger,
Vroom Thea M.
Publication year - 1986
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19860915)58:6<1252::aid-cncr2820580613>3.0.co;2-m
Subject(s) - transferrin receptor , transferrin , immunofluorescence , pathology , monoclonal antibody , receptor , immunohistochemistry , grading (engineering) , antibody , malignancy , lymphoma , medicine , microbiology and biotechnology , biology , immunology , ecology
A series of 34 B‐cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. There were diffuse centrocytic, diffuse centroblastic, and follicular and diffuse centrocytic/centroblastic lymphomas. Significant differences in log mean nuclear area in these four histologic groups were found, as well as a significant correlation between log nuclear area and transferrin receptors as measured semiquantitatively by fluorescence intensity of the cell membranes. In addition, it was found that there is a strong correlation between the transferrin receptor activity and malignancy grading on histologic and morphometric basis. Therefore, the transferrin receptor determination on frozen sections appears to be a good method of malignancy grading.