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Establishment of a human large cell lung tumor line (QU‐DB) with metastatic properties in athymic mice
Author(s) -
Cole SUSAN P. C.,
Roder John C.,
Campling Barbara G.,
Dexter David F.,
Holden JEANETTE J. A.
Publication year - 1986
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19860815)58:4<917::aid-cncr2820580419>3.0.co;2-0
Subject(s) - cell culture , metastasis , doubling time , pathology , cell , medicine , karyotype , lung , primary tumor , cancer research , biology , cancer , chromosome , biochemistry , genetics , gene
A continuous human cell line was established from a patient with large cell anaplastic lung carcinoma. This cell line, designated QU‐DB, has been in culture for over 36 months and grows as an adherent monolayer with a doubling time of 10–12 hours. Its morphology, ultrastructure, karyotype, ability to grow in soft agar and heterotransplantability, indicate it is a large‐cell lung tumor cell line of human origin. Three cell lines were established from metastatic tumors in nude mice receiving subcutaneous injections of QU‐DB cells. The morphology and growth characteristics exhibited by these cell lines were similar to the primary cell line. Karyotypic analysis of cell lines derived from the primary tumor and a metastasis to the diaphragm were similar, but cells from a liver metastasis culture showed additional karyotypic changes. This large cell lung tumor cell line may prove useful as a model system for studies of human tumor progression and metastasis.