Premium
Enzymes of purine metabolism in hairy cell leukemia
Author(s) -
Ho Anthony D.,
Dietz Günter,
Trede Irmtraut,
Schwartz Reinhard,
Hoffbrand A. Victor,
Hunstein Werner
Publication year - 1986
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19860701)58:1<96::aid-cncr2820580117>3.0.co;2-3
Subject(s) - adenosine deaminase , purine nucleoside phosphorylase , hairy cell leukemia , 5' nucleotidase , purine , chronic lymphocytic leukemia , purine metabolism , enzyme , leukemia , deoxycoformycin , nucleotidase , medicine , biochemistry , microbiology and biotechnology , immunology , chemistry , biology
Differences in activities of the purine degradative enzymes, adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP), and 5′‐nucleotidase (5′NT), have been observed among different classes of lymphoid malignancies. Recent studies have shown that hairy cell leukemia (HCL) may respond to treatment with the ADA inhibitor, 2‐deoxycoformycin. This study demonstrates that the cells of HCL have significantly lower levels of ADA and 5′NT (P always < 0.01) when compared to levels in normal B‐ or T‐lymphocytes, but have higher levels of PNP (P 0.001 for both comparisons). Recent studies have shown that when treated with 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), cells of B‐cell chronic lymphatic leukemia (B‐CLL) acquire phenotypic characters of HCL. The authors have therefore also investigated the changes in enzyme pattern of B‐CLL after incubation with TPA B‐CLL cells are characterized by low levels of ADA, PNP, and 5‐NT, but TPA caused a marked increase in PNP activity (P 0.001, t test for paired samples), a pattern similar to HCL. The results from biochemical studies are thus in accordance with the hypothesis that HCL cells are more mature than B‐CLL cells. The special enzyme profile of HCL suggests that a PNP inhibitor might also be effective in the treatment of this disease. Cancer 58:96–99, 1986.