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Immunohistologic identification of phenotypic antigens associated with Hodgkin and reed‐sternberg cells. A paraffin section study
Author(s) -
Sherrod Andy E.,
Felder Barbara,
Levy Norman,
Epstein Alan,
Marder Robert,
Lukes Robert J.,
Taylor Clive R.
Publication year - 1986
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19860601)57:11<2135::aid-cncr2820571109>3.0.co;2-n
Subject(s) - reed–sternberg cell , histiocyte , immunostaining , antigen , pathology , antibody , monoclonal antibody , immunohistochemistry , lymphoma , frozen section procedure , phenotype , biology , medicine , microbiology and biotechnology , immunology , hodgkin lymphoma , genetics , gene
A combination of two monoclonal antibodies, designated LN‐1 and LN‐2, were used in an attempt to identify the corresponding antigens in Hodgkin and Reed‐Sternberg cells. The LN‐1 antibody has been shown in previous studies in our laboratories to identify follicular center cells, whereas LN‐2 marks certain B‐cell subpopulations as well as interfollicular histiocytes. Utilizing the perioxidase‐antiperioxidase (PAP) technique, paraffin‐embedded sections were examined representing 39 cases of various histologic subgroups of Hodgkin's lymphoma following immunostaining with LN‐1, LN‐2, and the antibody to S‐100. Of these 39 cases, the LN‐2 antibody was found to consistently mark the majority of Hodgkin and Reed‐Sternberg cells. LN‐1 was found to identify Hodgkin and Reed‐Sternberg cells in a smaller number of cases. In no instance were Hodgkin or Reed‐Sternberg cells found to mark for the S‐100 protein. The use of LN‐1 and LN‐2 antibodies facilitated the identification of Hodgkin and Reed‐Sternberg cells and produced additional information regarding the phentotypic nature of these cells.