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Urothelial dysplasia and carcinoma in situ of the bladder
Author(s) -
Hofstädter Ferdinand,
Delgado Ricardo,
Jakse Gerhard,
Judmaier Werner
Publication year - 1986
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19860115)57:2<356::aid-cncr2820570228>3.0.co;2-7
Subject(s) - carcinoma in situ , urothelium , dysplasia , pathology , feulgen stain , in situ , staining , carcinoma , medicine , bladder cancer , dna , ploidy , cancer , urinary bladder , biology , chemistry , urology , genetics , organic chemistry , gene
Urothelial cells were pepsin‐extracted from paraffin‐embedded specimens taken from human nontumorous bladder mucosa, dysplasia, and carcinoma in situ . After Feulgen staining for DNA, nuclei were measured with an integrating microdensitometer. The measurements show that normal urothelium consists mostly of diploid nuclei. Dysplasia means that there is a predominance of tetraploid DNA values, whereas carcinoma in situ is characterized by a high percentage of aneuploid cells. In both dysplasia and carcinoma in situ there is a considerable percentage of diploid nuclei. Thus, DNA cytophotometry can be used for standardization of preneoplastic and early stages of tumor development in bladder cancer.