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Peaunut Agglutinin. A useful marker for histiocytosis x and interdigitating reticulum cells
Author(s) -
Ree H. J.,
Kadin Marshall E.
Publication year - 1986
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19860115)57:2<282::aid-cncr2820570216>3.0.co;2-q
Subject(s) - pathology , staining , histiocyte , histiocytosis , stain , malignant histiocytosis , peanut agglutinin , reticulum , antibody , microbiology and biotechnology , langerhans cell histiocytosis , medicine , biology , endoplasmic reticulum , immunology , lectin , disease
We studied the peanut agglutinin (PNA) staining patterns of histiocytosis X (H‐X) (three cases, including one case of Letterer‐Siwe disease) and well‐characterized cases of malignant histiocytosis (two cases) and monoblastic leukemia (one case) in paraffin‐embedded specimens. H‐X cells showed staining identical to that of interdigitating reticulum cells, with unique paranuclear and cell surface deposits of reaction products. Thus, H‐X cells were easily distinguished from benign macrophage‐histiocytes, which show diffuse cytoplasmic staining, and malignant cells of malignant histiocytosis and monoblastic leukemia, which did not stain with PNA. PNA staining proved more useful than Leu‐M1 antibody for this purpose, since neither interdigitating reticulum cells in ten reactive lymph nodes nor Langerhans' ‐type cells in three cases of H‐X were stained with Leu‐M1 antibody. These observations support a close relationship between H‐X cells and interdigitating reticulum cells, and demonstrate the usefulness of PNA for the identification of these cells in paraffin‐embedded material.