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Semiautomated measurement of rapidly hydrolyzed DNA in the diagnosis of mammary carcinoma
Author(s) -
Sincock Andrew M.
Publication year - 1986
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19860101)57:1<1::aid-cncr2820570103>3.0.co;2-t
Subject(s) - feulgen stain , breast carcinoma , medicine , carcinoma , dna , human breast , mammary carcinoma , interfacing , breast cancer , pathology , cancer , biology , computer science , staining , biochemistry , computer hardware
A modified Feulgen procedure combined with integrating microdensitometry was used to quantify the rapidly hydrolyzed DNA fractions in nuclei obtained from primary carcinomas of the human breast. The method, which was semiautomated by interfacing the microdensitometer with a Research machines 380Z microcomputer (Research machines, Ltd., Oxford, UK), could distinguish malignant carcinomas from benign breast lesions. It is proposed that the semiautomated assessment of rapidly hydrolyzed DNA fractions could serve as a reliable additional parameter on which to diagnose breast malignancies.