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Insulinoma. An immunocytochemical and morphologic analysis of 95 cases
Author(s) -
Liu TongHua,
Tseng HsienChiu,
Zhu Yu,
Zhong ShouXian,
Chen Jie,
Cui QuanCai
Publication year - 1985
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19850915)56:6<1420::aid-cncr2820560633>3.0.co;2-y
Subject(s) - pancreatic polypeptide , gastrin , somatostatin , vasoactive intestinal peptide , immunocytochemistry , motilin , medicine , endocrinology , glucagon , gastric inhibitory polypeptide , insulinoma , secretin , pancreas , delta cell , peptide hormone , biology , hormone , neuropeptide , secretion , receptor
One hundred twenty‐seven insulinomas from 95 cases (1 malignant and 94 benign) were studied pathologically. Thirty‐six tumors (35 cases) were examined by electron microscopy. Typical β‐cell secretory granules of crystalloid‐form cores and/or atypical secretory granules were discerned in all tumors examined. A new type of secretory granule, with high electron‐dense crystalloid‐form cores and moderate electron‐dense granular substance filling the space between the core and the limiting membrane, were observed in two cases. Among 68 insulinomas (67 cases) subjected to immunocytochemical investigations with ten peptide hormones (insulin, glucagon, somatostatin, pancreatic polypeptide (PP), gastrin, motilin, secretin, vasoactive intestinal polypeptide (VIP), gastric inhibitory polypeptide (GIP), and neurotensin), 42 were found to be multihormonal, varying from two to four peptides secreted. The hormones contained were insulin, glucagon, PP, somatostatin, and gastrin in different combinations. One patient had hyperinsulinemia and hypergastrinemia concurrently, and two islet tumors were excised at an interval of 10 months. Both electron microscopy and immunocytochemistry confirmed the presence of β‐ and α‐cells in the first tumor, whereas the second tumor revealed only G‐cells by electron microscopy, and G‐ and β‐cells on immunocytochemical staining. The morphologic and immunocytochemical characteristics of the insulinomas in this series are discussed.