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Cell‐cycle‐dependent expression of human melanoma membrane antigen analyzed by flow cytometry
Author(s) -
Leong Stanley P. L.,
Bolen James L.,
Chee Darwin O.,
Smith Vivian R.,
Taylor Joseph C.,
Benfield John R.,
Klevecz Robert R.
Publication year - 1985
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19850315)55:6<1276::aid-cncr2820550621>3.0.co;2-9
Subject(s) - antigen , flow cytometry , cell cycle , population , monoclonal antibody , microbiology and biotechnology , cell , biology , melanoma , immunofluorescence , antibody , cell culture , immunology , cancer research , medicine , biochemistry , genetics , environmental health
Knowledge of tumor antigenic expression is crucial to the design of therapeutic strategy. A murine monoclonal antibody (BE4) against a human melanoma membrane antigen, was used to study the in vitro expression of this antigen. By membrane immunofluorescence, BE4 reacted against 5 of 8 melanoma lines as compared to zero of 13 other cell populations. Using flow cytometry, the antigenic M14 CEM melanoma cells consisted of 40% to 60% of the total cell population. Dual‐parameter measurements of DNA content and membrane antigen demonstrated that the nonantigenic cells were predominantly in G0/G1 phase, whereas the antigenic cells were distributed throughout the cell cycle. Within one passage, the sorted and recultured nonantigenic population demonstrated a similar proportion of antigenic cells as the unsorted original population. It was concluded that the expression of human melanoma antigen was cell‐cycle‐dependent. Understanding factors that turn off the expression of antigen in G0/G1 phase may lead to better immunotherapeutic strategies. Cancer 55:1276‐1283, 1985.