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ARH‐77, an established human IgG‐producing myeloma cell line. I. morphology, B‐cell phenotypic marker profile, and expression of epstein‐barr virus
Author(s) -
Drewinko Benjamin,
Mars Wendy,
Minowada Jun,
Burk Kenneth H.,
Trujillo Jose M.
Publication year - 1984
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19841101)54:9<1883::aid-cncr2820540919>3.0.co;2-q
Subject(s) - phenotype , morphology (biology) , medicine , cell culture , virus , virology , cell , multiple myeloma , microbiology and biotechnology , immunology , biology , gene , genetics
Monolayer cultures of ARH‐77 cells, a human myeloma cell line propagated in vitro , display a variety of morphologic entities ranging from small lymphocytes to classic plasma cells. The cells show intense pyronin and periodic acid‐Schiff affinity but are negative for colloidal iron, sudan black, and naphtol AS‐D chloroacetate esterase. The cells exhibit phenotypic markers pertaining to each stage of the B‐cell lineage. They fail to display sheep erythrocyte and bovine erythrocyte—IgG antibody complex rosettes, common acute lymphocytic leukemia (ALL) antigens and T‐cell antigens, but most cells display surface complement receptors, Ia‐like antigens, and surface and intracytoplasmic Ig. Monoclonal antibodies were negative for T‐antigens, myelomonocytic cell antigens, leukemia‐associated antigens, and BA‐1 and OKT‐10 antigens. However, 100% of the cells were positive with OKT‐9 and B3/25 antibodies that are specific for transferrin receptors. About 50% to 80% of the cells were positive for surface membrane immunoglobulin ( k IgG) and about 10% to 50% for cytoplasmic immunoglobulin ( k IgG). Virtually all cells were positive when tested for nuclear Epstein‐Barr virus antigens.