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Childhood acute lymphoblastic leukemia with natural killer activity. Clinical and cellular features of three cases
Author(s) -
Komiyama Atsushi,
Yamada Sachihiro,
Kawai Hiroshi,
Miyagawa Yukiaki,
Akabane Taro
Publication year - 1984
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19841015)54:8<1547::aid-cncr2820540814>3.0.co;2-#
Subject(s) - k562 cells , antigen , immunology , leukemia , natural killer cell , lymphoblast , raji cell , acute lymphocytic leukemia , biology , lymphocyte , cell , microbiology and biotechnology , medicine , cytotoxic t cell , cell culture , lymphoma , lymphoblastic leukemia , in vitro , biochemistry , genetics
Leukemic cells from 3 of 28 children with acute lymphoblastic leukemia (ALL) had natural killer (NK) activity against K562 and Molt‐3, but not against Raji as determined by the 4‐hour 51 Cr release assay at a 40:1 effector:target ratio; the highest percent lysis against K562 in each patient ranged from 55.9% to 147.4% of the normal lymphocyte value and that against Molt‐3 from 28.0% to 127.9%. Their leukemic cells were nonphagocytic, nonadherent, and negative for nonspecific esterase. Leukemic cells from two of them displayed similar morphologic and immunologic features. The cells had a round nucleus, and were E‐receptors (E) + , Leu‐5 + , IgG‐Fc + , Ia + , OKMl + or OKM1 − , T‐cell antigens − , B markers − , and monocyte antigen − , indicating their NK cell origin. The other patient's cells were characterized by the irregularly shaped nucleus, and were E + , Leu‐5 + , IgG‐Fc + , T + , OKT6 + , Leu‐2a + , and OKMl + . The presence of E which are identical to E of T‐cells and a high density of T‐cell antigens including OKT6 on the cells suggested their T‐cell nature. Of the three patients, two without thymic enlargement had leukemic cells of NK cell origin, and the other one with the symptom leukemic cells of the T‐cell lineage, the clinical feature probably reflecting the cell lineage of their leukemic cells.