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Acute megakaryoblastic leukemia in childhood
Author(s) -
Sariban Eric,
Oliver Constance,
Corash Lawrence,
Cossman Jeffrey,
WhangPeng Jackie,
Jaffe Elaine S.,
Gralnick Harvey R.,
Poplack David G.
Publication year - 1984
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19841001)54:7<1423::aid-cncr2820540732>3.0.co;2-0
Subject(s) - acute megakaryoblastic leukemia , cytochemistry , pathology , golgi apparatus , myeloperoxidase , medicine , leukemia , precursor cell , peroxidase , ultrastructure , microbiology and biotechnology , endoplasmic reticulum , biology , cell , immunology , enzyme , biochemistry , inflammation
Routine morphologic and cytochemical study of the leukemic cells of a 13‐month‐old child did not permit definitive determination of either a lymphoid or a myeloid cell origin. However, ultrastructural cytochemical analysis revealed platelet peroxidase (PPO) reactivity. The malignant cells expressed PPO in both unfixed and fixed preparations. PPO was observed in the perinuclear space and reticulum but not in the Golgi saccules. In a 1% glutaraldehyde, 1% formaldehyde solution known to inhibit PPO but not myeloperoxidase, no reaction product was observed. The demonstration of PPO activity in these undifferentiated cells established their megakaryoblastic lineage. Further studies including DNA flow cytometry confirmed that these cells were megakaryoblasts. This study demonstrates that megakaryoblastic malignancy may occur as an acute leukemia of childhood and emphasizes the value of ultrastructural cytochemistry in cases of acute leukemia which defy routine classification. Abnormalities of chromosome 21 were present in our patient as in four previous cases of megakaryoblastic leukemia in childhood. The nonrandomness of chromosome 21 involvement in this disease should therefore be considered.