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Characterization of two patients with lymphomas of large granular lymphocytes
Author(s) -
Pandolfi Franco,
Pezzutto Antonio,
De Rossi Giulio,
Pasqualetti Daniela,
Semenzato Gianpietro,
Quinti Isabella,
Ranucci Alessandra,
Raimondi Roberto,
Basso Giuseppe,
Strong Douglas M.,
Fontana Luigi,
Aiuti Fernando
Publication year - 1984
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19840201)53:3<445::aid-cncr2820530313>3.0.co;2-d
Subject(s) - antibody dependent cell mediated cytotoxicity , peripheral blood mononuclear cell , monoclonal antibody , immunology , phenotype , lymphoma , in vitro , antibody , biology , suppressor , receptor , monoclonal , cancer research , cancer , biochemistry , genetics , gene
Two patients with non cutaneous well‐differentiated lymphocytic lymphoma with leukemic spread are reported. The large majority of their peripheral blood mononuclear cells (PBMC) formed rosettes with sheep erythrocytes, had receptors for the Fc portion of IgG, and an enzymatic profile of relatively mature T‐cells. These cells were morphologically characterized as large granular lymphocytes. Studies with monoclonal antibodies in one of the cases showed an OKT3+, OKT10‐, OKT4‐, OKT8‐, HNK‐1‐, OKM1+ phenotype, whereas PBMC from the other case were OKT3+, OKT10‐, OKT4‐, OKT8+, HNK‐1+, OKM1‐. PBMC from the first patient were able to suppress in vitro B‐cell differentiation and were capable of a strong antibody dependent cellular cytotoxicity (ADCC) activity. Natural killer (NK) activity was reduced. Cells from the other patient who was hypogammaglobulinemic, exerted suppressor activity in immunoregulatory assays, and showed ADCC and NK activity. These data support the existence of LGL lymphomas consisting of the proliferation of mature appearing cells capable of functional activity.