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Reactivity of monoclonal antibodies leu 1 and OKT1 with malignant human lymphoid cells. Correlation with conventional cell markers
Author(s) -
Knowles Daniel M.,
Halper James P.,
Azzo Walid,
Wang Chang Yl
Publication year - 1983
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19831015)52:8<1369::aid-cncr2820520806>3.0.co;2-3
Subject(s) - pathology , monoclonal antibody , antigen , antibody , malignant transformation , isotype , calla , b cell , monoclonal , follicular lymphoma , lymphoma , biology , medicine , microbiology and biotechnology , immunology
This study delineated the distribution of reactivity of malignant human lymphoid cells with monoclonal antibodies Leu 1 and OKT1, and correlated this expression with that of conventional lymphoid cell markers. The presence of Leu 1 on benign lymph nodal T‐cells and its absence from benign lymph nodal B‐cells was confirmed. Twenty‐two T‐cell neoplasms, expressing a variety of intrathymic and mature peripheral phenotypes, expressed Leu 1, but this expression was heterogeneous with respect to percent‐positive cells and antigenic density, and appeared to correlate with stages of T‐cell differentiation. This study demonstrated the expression of Leu 1 by 33 of 36 cases of B‐CLL, by 10 of 15 cases of the closely allied small lymphocytic cell lymphoma, and by 9 of 29 follicular center‐cell lymphomas. This included B‐cell malignancies of each surface immunoglobulin isotype, and some cases associated with a monoclonal protein spike. Leu 1 was not expressed by myeloma plasma cells, and was absent from non‐B, non‐T acute lymphoblastic leukemia cells in each of 15 cases studied. Finally, Leu 1 and OKT1 were expressed in parallel, with respect to percent‐positive cells and staining intensity, on benign and malignant T‐cells, and on malignant B‐cells, wherever studied. Possible explanations for this shared antigen are the existence of a minor Leu 1 + B‐cell subset, a transformation‐associated event, or glycosylation.