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Proliferative activity of bone marrow cells in primary dysmyelopoietic (preleukemic) syndromes
Author(s) -
Montecucco Carlomaurizio,
Riccardi Alberto,
Traversi Egidio,
Giordano Paolo,
Mazzini Giuliano,
Ascari EDOARDO
Publication year - 1983
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19831001)52:7<1190::aid-cncr2820520711>3.0.co;2-r
Subject(s) - bone marrow , medicine , acute myelomonocytic leukemia , pathology , leukemia , anemia , population , karyotype , myelodysplastic syndromes , acute leukemia , immunology , cancer research , biology , chromosome , genetics , environmental health , gene
The proliferative activity of bone marrow cells was studied in 24 patients with primary dysmyelopoiesis by means of flow cytometry and 3H‐IdR autoradiography. Abnormal DNA content was found in two cases with aneuploid karyotypes. DNA content typical of a diploid population was observed in all patients with normal karyotype and in three patients with chromosomal aberrations. The fraction of bone marrow cells in S‐and G 2 ‐phase was higher in primary acquired sideroblastic anemia and refractory anemia (without excess of blasts) than in refractory anemia with excess of blasts and chronic myelomonocytic leukemia. Regardless to the diagnosis, the patients with low fraction of cells in S‐ and G 2 ‐phase had short survival time and showed high rate of evolution into acute nonlymphoblastic leukemia. The labeling (LI) and mitotic (MI) indexes of both erythroblasts and granulocytic cells were decreased in nearly all patients. The lowest values of LI and MI of the granulocytic compartment were found in the patients who subsequently developed acute leukemia. These data suggest that cytokinetic analysis allows investigators to achieve useful information on the stage of disease in the dysmyelopoietic syndromes.