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In vitro sensitivity of normal granulocytic and lymphoma colonies to vinca alkaloids
Author(s) -
Smith Stephen D.,
Trueworthy Robert C.,
Kisker Steven E.,
Noller Larry G.,
Lowman James T.
Publication year - 1983
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19830201)51:3<417::aid-cncr2820510310>3.0.co;2-w
Subject(s) - vinca , vinblastine , vinca alkaloid , vindesine , in vitro , vincristine , pharmacology , alkaloid , medicine , colchicine , antineoplastic drugs , biology , chemotherapy , biochemistry , botany , cyclophosphamide
Recently, a colony‐forming assay was developed in our laboratory for pediatric malignant lymphoid diseases. This assay supports the growth of lymphoma colonies (ML‐CFC) as well as normal granulocytic colonies (CFU‐C) and thus a direct comparison between the antineoplastic and myelosuppressive effects of a drug can be determined. To test specificity of this in vitro assay to structurally similar drugs, the inhibitory effects of three vinca alkaloids (vincristine, vindesine, vinblastine) on ML‐CFC (B‐, T‐, pre‐T‐cell types) and CFU‐C was determined. Our results demonstrate that all three vinca alkaloids were active agents in vitro and that a direct dose response effect occurred once a threshold dose was reached. Each vinca alkaloid had a different pattern of inhibitory effect on ML‐CFC and CFU‐C suggesting an inherent difference in drug metabolism by these cells. Also, based on the dose inhibiting 50% of colony formation, vinblastine was 94 times more inhibitory against malignant B‐cell ML‐CFCs than against granulocytic CFU‐C.